Description:This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn: How RNAi works in worms, flies and mammalsHow to design the most efficient RNAi constructsHow to achieve transient, stable and conditional RNAi in cell culturesHow to determine the efficiency of an RNAi experimentand how to use RNAi for gene therapyAll the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.We have made it easy for you to find a PDF Ebooks without any digging. And by having access to our ebooks online or by storing it on your computer, you have convenient answers with RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. Elegans, Drosophila, and Mammals. To get started finding RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. Elegans, Drosophila, and Mammals, you are right to find our website which has a comprehensive collection of manuals listed. Our library is the biggest of these that have literally hundreds of thousands of different products represented.
Pages
336
Format
PDF, EPUB & Kindle Edition
Publisher
Wiley-Vch
Release
2006
ISBN
3527604375
RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. Elegans, Drosophila, and Mammals
Description: This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn: How RNAi works in worms, flies and mammalsHow to design the most efficient RNAi constructsHow to achieve transient, stable and conditional RNAi in cell culturesHow to determine the efficiency of an RNAi experimentand how to use RNAi for gene therapyAll the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.We have made it easy for you to find a PDF Ebooks without any digging. And by having access to our ebooks online or by storing it on your computer, you have convenient answers with RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. Elegans, Drosophila, and Mammals. To get started finding RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. Elegans, Drosophila, and Mammals, you are right to find our website which has a comprehensive collection of manuals listed. Our library is the biggest of these that have literally hundreds of thousands of different products represented.